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2024-03-17 06:51:17
Gibco | Thermo Fisher Scientific - PH
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Cell Culture MediaCell Culture ReagentsCell Culture PlasticsCell Therapy Systems (CTS)Cell Culture SeraCancer Cell CultureProtein Expression SystemPrimary Cell Culture
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Solutions for the everyday and the extra challenging
From the first time you culture cells and throughout your career journey, we can connect you with the trusted culture formulations that match your experiments and a portfolio of proven, scalable solutions to help deliver your products to market.Gibco media, sera, supplements, cells, cell culture reagents and talented support teams are all designed to help empower your work. And our technical, scientific and regulatory professionals are always available to help you keep your work moving forward.Cell & tissue cultureCancer cell cultureCell therapy systemsProtein expression systemsBioprocessingGibco education
Gibco video library
Cell Culture Basics Education
Cell Culture Media and Reagents
Cell Culture Supplement - FBS
Cell Therapy Systems – Xenon Electroporation System
Cell Therapy Systems - Rotea Counterflow Centrifugation System
Protein Expression Systems
xxYou probably think you know Gibco. But, do you?From research to production, and starter kits to commercialization, our online resources and technical support teams empower your end-to-end journey.We celebrated the 60th anniversary of Gibco cell culture across the globe with everyone who makes it special, you! To commemorate this celebration, we captured the amazing moments and wonderful stories along the way.Gibco Cell Culture at 60 x
Love Your Cells
Celebrate the connection between scientists and their cellsxSome of life’s biggest challenges can be solved with some of life’s smallest things: cells. We have asked a small group of scientists to record love letters, written in their own words, exploring the extraordinary relationships they have with their cells. Join our celebration of a connection like no other as we delve into a love story told through science and art.Watch more love letter videos Be a Cell Culture HeroWe are proud to celebrate the science and celebrate you. Cell Culture Heroes connects PhD and postdoc researchers from around the world through inspirational posts and engaging webinars, shining a light on their amazing stories, cutting-edge work and game-changing results.Sustainable solutionsConnect with more environmentally sustainable science. We’ve developed over 50 product lines that can meet your precise needs in the lab and don’t sacrifice on performance, while having a less harmful impact on the environment.Key applications
Mammalian cell cultureGibco media, sera, and reagents alongside Nunc Cell Culture Plastics and Invitrogen Transfection Reagents, comprise an end-to-end offering to support your mammalian cell culture needs.Primary cell culturePrimary cell culture systems optimized for each cell typeBioprocessingCustom Gibco cell culture media and services for developers and manufacturers of biologicsStem cell cultureGibco cells, media, growth factors, and supplements for stem cell cultureCancer cell cultureGibco media, sera, and reagents for cancer cell lines, spheroids and organoidsOrganoids, spheroids & 3D cell cultureOrganoids, spheroids, and the study of cells as 3D models show great potential in many applications including disease modeling and regenerative medicineCell therapy systemsMedia, supplement, and growth factors for cell therapy applicationsDrug developmentGibco primary hepatocytes, media, and supplements offer the stable foundation you need to more confidently screen compounds for potential effects to liver functionCytogeneticsGibco media and supplements optimized for clinical cytogenetic protocolsNeurobiologyStreamline your neurobiology experiments, and obtain reliable results with trusted Gibco products and technologiesProtein expressionEasy-to-use protein expression systems that deliver high protein yields for every downstream applicationExtracellular matrices (ECM)Our Gibco extracellular matrices, scaffolds, and proteins provide in vivo–like morphology and physiologically relevant environments for more realistic cell biology and better intercellular interactions.
Resources
Gibco Cell Culture Basics Resource HubThis rich content hub can get you started on your way to confident cell culture with handbooks, videos and virtual labs.Cell culture workflowFrom culture to discovery, every passage, every step, you’re covered.Cell lines databaseGibco cell culture companion mobile appMedia FormulationsProduct FAQsProduct support documentation (COA, formulations, etc...)ProtocolsLook for Gibco on: Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.
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Home›Life Sciences›Cell Culture & Transfection›Mammalian Cell Culture›Cell Culture MediaCell Culture MediaShop all basal media Media formulation toolSee Navigation‹Mammalian Cell CultureFetal Bovine Serum (FBS)
›Cell Culture Media
Dulbecco's Modified Eagle Medium (DMEM)
Dulbecco's Modified Eagle Medium F12 (DMEM F12)
Minimum Essential Medium (MEM)
Roswell Park Memorial Institute (RPMI) 1640 Medium
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›Gibco Cell Culture Media for healthy mammalian cell culturesCell culture medium is vital to your culture environment as it provides components (e.g., nutrients, growth factors) necessary to support cell growth and function. In addition, media can be formulated to regulate environmental conditions in cell culture, including osmotic pressure and pH. A variety of cell culture media formulations have been developed to support a wide range of cell types and experimental applications.Time-tested and trusted, our Gibco cell culture media line includes products designed to support the growth and maintenance of a variety of mammalian cells and cell lines. We offer ready-to-use cell culture media as well as powdered and concentrated liquid formulations to fit your experimental setup and budget. For your scale-up needs, Gibco Cell Culture for Bioprocessing provides end-to-end products, process development and customization services to help maximize productivity while reducing costs and time to market.
Learn how Gibco Media are the most cited media in global scientific journals
Gibco cell culture media are a reliable solution, designed to deliver reproducibility and performance, for results you can count on. View cell culture brochure Click image to enlarge
Explore classical media
DMEMDulbecco’s Modified Eagle Medium (DMEM), a widely used cell culture medium, is more nutrient-rich than MEM and is proven for use with many mammalian cell types.Explore Gibco DMEMRPMI 1640Roswell Park Memorial Institute (RPMI) 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins.Explore Gibco RPMI 1640DMEM F12Dulbecco's Modified Eagle Medium Nutrient Mixture F-12 (DMEM F12), a 1:1 mixture combining the benefits of DMEM with the added nutrients found in Ham's F-12.Explore Gibco DMEM F12MEMMinimum Essential Medium (MEM), a commonly used cell culture media, can be modified to support a variety of mammalian cell culture types and applications.Explore Gibco MEMSelecting the right cell culture mediaThe success of any cell culture experiment depends on choosing the optimal cell culture media formulation for your unique cells and experimental conditions. Use the selection tool to choose from all options including classical media and innovative formulations.Use our interactive Cell Culture Media Formulation Tool to compare and modify basal media to best fit your lab’s needsInnovative cell culture media formulationsWe are committed to innovating Gibco cell culture media. We go beyond basic formulations to provide optimized and innovative cell culture solutions in support of your efforts to achieve consistently reproducible results.BenchStable MediaGibco BenchStable Media, available in our most common cell culture media formulations, are stable at room temperature, allowing for convenient and flexible storage without utilizing cold storage space.Explore Gibco BenchStable MediaHuman Plasma-Like MediumGibco Human Plasma-Like Medium (HPLM) is formulated to mimic the metabolic profile of human plasma, allowing for improved physiological relevance when studying cancer and disease.Explore Gibco Human Plasma-Like MediumMedia with GlutaMAX SupplementMedia with Gibco GlutaMAX Supplement are standard cell culture formulations containing a stabilized form of L-glutamine that prevents degradation and buildup of ammonia.Explore GlutaMAX Media formulations
Learn more about our commitment to innovation
Constant innovation for cutting-edge cell culture, from our research labs to yoursYou need a partner dedicated to helping you confidently forge new paths. Our team of skilled R&D scientists and our unwavering investment in innovation make Gibco cell culture media the trusted choice of boundary-pushing scientists around the world.Other cell culture mediaGibco cell culture media are available in a variety of formulations to suit your research needs. Explore these additional options below.IMDM mediaGibco Iscove’s Modified Dulbecco’s Medium (IMDM) is a highly enriched medium well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. Choose from a large selection of IMDM media compositions for a range of cell culture applications.
Order IMDM media
31980030,31980097,21056023,12440053,12440046,12440061,12440079,12200069,12200036, 21980032,21980065,42200014,42200030Media 199Gibco Media 199 (M199 media) is useful across a wide range of species and applications but is particularly suitable for the culturing of non-transformed cells. M199 medium ingredients may include Earle’s salts and a bicarbonate/carbonic acid buffering system for the maintenance of pH in a CO2 incubator or Hank’s salts with salt solutions designed for atmospheric equilibrium.
Order Media 199
11150059, 11150067,31150022,21180021,31100019,31100035,12340030,22340020,22340087,31150030, 31153026,12350039, 22350029,22350078,10012011,11825015,11043023,21157029,31100027Ham’s F-12Gibco Ham’s F-12 Nutrient Mixture was formulated for single-cell plating of near-diploid Chinese Hamster Ovary (CHO) cells but has also been used for serum-free growth of CHO cultures and serum-supplemented growth of mammalian cells. Select from several high-quality and reliable formulations of Ham’s F-12 media, including glutamine-supplemented F-12 medium as well as GlutaMAX supplemented formulations, Kaighn's formulation, and powdered formats.
Order Ham’s F-12
31765035,31765092,11765047,11765054,11765062,11765070,21127022,21127030,21700018,21700075,21765037,21765029,21700026Ham’s F-10Gibco Ham’s F-10 Nutrient Mixture is formulated for serum-free growth of Chinese Hamster Ovary (CHO) cells and the growth of various mammalian cell lines with serum supplementation. Rely on Gibco Ham's F-10 medium for superior quality, greater reliability, and improved consistency to give you better overall efficiency and more robust data.
Order Ham’s F-10
11550043,12390035,41550021,31550031,31550049,31550023,31550015Custom cell culture mediaCustomize Gibco media catalog products—add or remove components, create customized packaging options, select QC tests, and more. Media formulations available for customization include classical basal media, protein expression media, stem cell media, and cell and gene therapy (CGT) media. Download the Gibco Custom media flyerExplore media customization services Cell culture media for bioprocessingPartnering with you to empower progress in bioprocessing around the world. Whether you are developing a vaccine or need help scaling up, our dedicated team will keep you at the forefront of the evolving bioprocessing industry.Explore scale-up opportunities
Additional cell culture media
Hybridoma mediaInsect mediaMicrobiology mediaCell freezing mediaBioprocessing mediaBasal Medium Eagle (BME)Fischer's MediumGlasgow's MEM (GMEM)Leibovitz's L-15 MediumLeibovitz's L-15 (GlutaMAX)Leibovitz's L-15 (no phenol red)Leibovitz's L-15 Medium, powderMcCoy's 5A (GlutaMAX)McCoy's 5A (HEPES)Lab Armor BeadsGibco bottle weightGibco media bottlesGibco media bagsCell culture media by applicationRegardless of whether you are performing cell culture in a research lab or production facility, need a special or defined formulation, or are growing cell lines, primary cells, or stem cells, Gibco products offer a reliable solution.Organoid, spheroid, and 3D cultureGibco media products have been used in a range of organoid, spheroid, and 3D culture studies, allowing for a more physiologically relevant understanding of complex biology.Explore media for organoid, spheroid, and 3D cell culture Stem cell cultureAn extensive selection of high-quality media products are available for pluripotent stem cells, neural stem cells, mesenchymal stem cells, hematopoietic stem cells, and stem cell therapy applications.Explore media for stem cell cultureNeuronal cell cultureGibco neuronal and neural cell media are optimized for the growth and support of primary neural cell and neuronal cultures and have been cited in applications involving primary neurons, glial cells, and disease models.Explore media for neuronal cell culturePrimary cell cultureA wide range of Gibco media products are available for the support of many primary cell types, including fibroblasts, hepatocytes, and many more.Explore media for primary cell cultureCell Therapy SystemsGibco Cell Therapy Systems (CTS) media products, designed for use in cell therapy applications, are manufactured to GMP standards and undergo extensive testing to ensure sterility and safety.Explore CTS mediaProtein expression mediaSpecific Gibco media formulations are available as components of our protein expression systems, supporting the generation of recombinant proteins for further use, including functional and therapeutic studies.Explore media for protein expressionMedia for cytogenetic research A selection of specially formulated Gibco media are available for use in standard clinical cytogenetic protocols, with products optimized for analysis of amniotic fluid cells, chronic villus samples, bone marrow cells, or peripheral blood lymphocytes.Explore media for cytogenetic researchSerum-free mediaGibco serum-free media are formulated with specific combinations of nutrients and hormones that support the growth of certain cells in the absence of animal sera, allowing for increased selectivity of the media, easier purification, and more consistent performance.Explore Gibco serum-free media
Cell culture media best practices
Here are a few simple tips & tricks to help ensure your cell culture media are maintained for optimal performance. If refrigerated, warm your cell culture media before use. Your cells would be cold at 2–8°C, and you would be too! Keep your cell culture media protected from light. Light exposure degrades the essential vitamins in media that your cells need to grow. Once you have supplemented the cell culture media with FBS, place the complete media into the refrigerator (2–8°C ) to maintain performance. Use your supplemented media within 2–4 weeks to reduce the chances of contamination and the impact of pH drift. GMP manufacturingGibco cell culture products are manufactured in facilities compliant with current good manufacturing practices (GMP) and adhere to a robust quality management system, helping to ensure the consistency, reliability, and high quality you can rely on.Learn more about GMP manufacturing Sustainable solutionsThe 1 L Gibco bottle uses 38% less source material (32% less material for the 500 mL bottle) than comparable media bottles on the market today. This helps reduce the consumption of non-renewable resources, helping shrink your footprint.Download the Green Fact SheetLearn more about sustainable solutionsFor Research Use Only. Not for use in diagnostic procedures.Stylesheet for Classic Wide Template adjustments
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Home›BrandsGibcoSee Navigation
Popular Products
Cell Culture MediaCell Culture ReagentsCell Culture PlasticsCell Therapy Systems (CTS)Cell Culture SeraCancer Cell CultureProtein Expression SystemPrimary Cell Culture
Key Applications
Cell Culture WorkflowDrug DevelopmentGenome Editing SolutionsGibco for BioprocessingMammalian Cell CultureNeurobiologyTransfection Solutions
Resources
Gibco Cell Culture BasicsGibco Education
Additional Information
Life ScienceRelated Brands
Solutions for the everyday and the extra challenging
From the first time you culture cells and throughout your career journey, we can connect you with the trusted culture formulations that match your experiments and a portfolio of proven, scalable solutions to help deliver your products to market.Gibco media, sera, supplements, cells, cell culture reagents and talented support teams are all designed to help empower your work. And our technical, scientific and regulatory professionals are always available to help you keep your work moving forward.Cell & tissue cultureCancer cell cultureCell therapy systemsProtein expression systemsBioprocessingGibco education
Gibco video library
Cell Culture Basics Education
Cell Culture Media and Reagents
Cell Culture Supplement - FBS
Cell Therapy Systems – Xenon Electroporation System
Cell Therapy Systems - Rotea Counterflow Centrifugation System
Protein Expression Systems
xxYou probably think you know Gibco. But, do you?From research to production, and starter kits to commercialization, our online resources and technical support teams empower your end-to-end journey.We celebrated the 60th anniversary of Gibco cell culture across the globe with everyone who makes it special, you! To commemorate this celebration, we captured the amazing moments and wonderful stories along the way.Gibco Cell Culture at 60 x
Love Your Cells
Celebrate the connection between scientists and their cellsxSome of life’s biggest challenges can be solved with some of life’s smallest things: cells. We have asked a small group of scientists to record love letters, written in their own words, exploring the extraordinary relationships they have with their cells. Join our celebration of a connection like no other as we delve into a love story told through science and art.Watch more love letter videos Be a Cell Culture HeroWe are proud to celebrate the science and celebrate you. Cell Culture Heroes connects PhD and postdoc researchers from around the world through inspirational posts and engaging webinars, shining a light on their amazing stories, cutting-edge work and game-changing results.Sustainable solutionsConnect with more environmentally sustainable science. We’ve developed over 50 product lines that can meet your precise needs in the lab and don’t sacrifice on performance, while having a less harmful impact on the environment.Key applications
Mammalian cell cultureGibco media, sera, and reagents alongside Nunc Cell Culture Plastics and Invitrogen Transfection Reagents, comprise an end-to-end offering to support your mammalian cell culture needs.Primary cell culturePrimary cell culture systems optimized for each cell typeBioprocessingCustom Gibco cell culture media and services for developers and manufacturers of biologicsStem cell cultureGibco cells, media, growth factors, and supplements for stem cell cultureCancer cell cultureGibco media, sera, and reagents for cancer cell lines, spheroids and organoidsOrganoids, spheroids & 3D cell cultureOrganoids, spheroids, and the study of cells as 3D models show great potential in many applications including disease modeling and regenerative medicineCell therapy systemsMedia, supplement, and growth factors for cell therapy applicationsDrug developmentGibco primary hepatocytes, media, and supplements offer the stable foundation you need to more confidently screen compounds for potential effects to liver functionCytogeneticsGibco media and supplements optimized for clinical cytogenetic protocolsNeurobiologyStreamline your neurobiology experiments, and obtain reliable results with trusted Gibco products and technologiesProtein expressionEasy-to-use protein expression systems that deliver high protein yields for every downstream applicationExtracellular matrices (ECM)Our Gibco extracellular matrices, scaffolds, and proteins provide in vivo–like morphology and physiologically relevant environments for more realistic cell biology and better intercellular interactions.
Resources
Gibco Cell Culture Basics Resource HubThis rich content hub can get you started on your way to confident cell culture with handbooks, videos and virtual labs.Cell culture workflowFrom culture to discovery, every passage, every step, you’re covered.Cell lines databaseGibco cell culture companion mobile appMedia FormulationsProduct FAQsProduct support documentation (COA, formulations, etc...)ProtocolsLook for Gibco on: Intended use of the products mentioned on this page vary. For specific intended use statements please refer to the product label.
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Gibco Cell Culture for Bioprocessing
Gibco Cell Culture for Bioprocessing
Join forces with a trusted scalable bioprocessing partner
Celebrating 60 Years of Defining Moments in Cell Culture. Since 1962, we have been empowering progress in bioprocessing around the world. Our pioneering end-to-end products, process development and customization services can help maximize productivity while reducing costs and time to market. Whether you are developing a vaccine or need help scaling up, our dedicated team will keep you at the forefront of the evolving bioprocessing industry.
Contact us
Gibco CHO Media
We offer high-performing CHO media designed to optimize your workflow, including media that is chemically defined, animal origin–free, serum-free, and more.
Optimize your cell culture performance
Custom and Media Manufacturing Services
We can manufacture customized media or your formulation for production, provide non-GMP media prototyping, and create custom packaging, supporting your entire media manufacturing journey.
Streamline your manufacturing
Gibco Peptones
Enhance performance and reduce serum with our extensive portfolio of animal origin and animal origin–free peptones that can supplement a variety of cell culture media.
Enhance your bioprocess
Gibco Media by Design Services
Discover our range of customized solutions and services to help empower you to meet your project goals. From antibodies to advanced therapies, collaborate with our knowledgeable and experienced team.
Achieve success by design
Media Formats
From liquid and dry powder media to our innovative, proprietary Advanced Granulation Technology (AGT) dry format, discover the media formats that can accelerate your workflow.
Find your optimal format
Virus Expression
Browse our catalog of high-performing media, feeds, and supplements to support your vaccines and viral vector needs, such as our innovative serum-free and serum-reduced media.
Streamline your vaccine production
Feeds and Supplements
Get the most out of your cells and learn more about our range of feeds and supplements that can enhance your cell culture up to a 2- to 5-fold increase over batch culture.
Enhance your media and feed process
Stable Cell Line Development
Move your workflow from cell line to process development with cGMP compliant products that can simplify your workflow and accelerate your process to commercial manufacture.
Find solutions for your development needs
Cell Culture Serum
Trusted by customers worldwide as a result of its high quality, reliability, traceability, and award-winning support, learn where Gibco serum can support your processes.
Optimize your biopharmaceutical manufacturing requirements
Functional Additives
Designed to enhance your process development from beginning to end, discover the functional additives that can accelerate your bioprocessing workflow.
Learn how to accelerate your process development from beginning to end
Cell Therapy
Advancing your cell therapy product to clinical applications requires careful material selection and thoughtful process development.
Explore our portfolio
Gene Therapy
With our flexible gene therapy solutions, we can help you scale up as you grow for clinical and cGMP manufacturing.
Discover our solutions
Gibco CTS DynaCellect
A closed, automated isolation, activation, and bead removal system for cell therapy development and manufacturing.
Automate your cell therapy manufacturing
Facility Tours
Learn more about our facilities and take a 360° tour of any one of our cell culture media manufacturing sites.
Take a tour
Secure your supply of Advanced Granulation Technology (AGT) products - Immediate shipping now available
AGT is the most advanced, scalable, dry powder media format available for cell culture on the market today.
Learn more
Featured resources
Application notes
A collaborative study for understanding trace element variability in custom cell culture media
Efficient-Pro Medium and Feeds improve productivity in CHO-K1, CHO-S, and DG44 cells
Viral Vector HEK Media Panel addresses HEK293 cell lineage diversity in AAV production through basal screening
Improving Allogeneic Workflow and Cell Proliferation with CTS OpTmizer Pro SFM
Flyers
Gibco Media and Feeds Selection Guide
Scaling up with Gibco media
Gibco rapid prototyping service
Dynamis Scale-Up
Limiting the Use of Serum in Vaccine Processes
Podcasts
Implementing Continuous Perfusion Bioprocessing
Microbial Bioproduction
Multi-Omics in Media Development
Inside Bioprocessing Manufacturing of Gene Therapies
Webinars
Multi-Omics and Bioinformatics in Cell Culture Media Design
Using Peptones to Achieve Diverse and Demanding Bioproduction Goals
Impact of Media and Supplements on Monoclonal Antibody Quality Attributes
White papers
Trace element variation for chemically defined cell culture media
Transient protein expression ExpiCHO
Understanding perfusion terminology
Perfusion process selection
Perfusion hardware needs
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Home›Life Sciences›Bioprocessing›Gibco Cell Culture for Bioprocessing›Gibco Cell Therapy Scale Up Solutions›Gibco Cell Therapy Bioprocessing ReagentsGibco Cell Therapy Bioprocessing ReagentsSee Navigation‹Gibco Cell Therapy Scale Up SolutionsGibco Cell Therapy Bioprocessing Reagents
›Gibco Cell Therapy Bioprocessing Media
›Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and/or expansion of T-cells ex vivo. Whether working with polyclonal T-cells, antigen-specific T-cells, gene-modified T-cells, regulatory T-cells, or other T-cell subtypes, CTS Dynabeads magnetic beads enable the recovery of T-cells with properties comparable to in vivo–activated T-cells.Contact usOur Gibco CTS Detachable Dynabeads beads possess a proprietary active release mechanism—the first of its kind for GMP, clinical trial, and commercial manufacturing use. These new offerings are intended for use with the CTS DynaCellect system to achieve its full range of benefits. With its active release mechanism and high specificity, our CTS Detachable Dynabeads platform allows for significant flexibility and scalability of manufacturing processes that vary widely from one therapy to another. Its performance exhibits consistent and optimal isolation, activation, and purity of target T-cell populations while addressing the biological variability that cell therapy manufacturers are facing.The CTS Detachable Dynabeads beads are based on our existing paramagnetic bead technology, but conjugated to highly specific, single-domain VHH antibodies that target specific clusters of differentiation (CD) markers for isolation and/or activation. Each kit comes with an active release buffer enclosed in a CTS DynaCellect system-compatible bioprocess container. After isolation of target immune cells and magnetic separation in the CTS DynaCellect system, the release buffer allows a user to actively detach target cells from the CTS Dynabeads beads through a competitive binding mechanism. This flexible and scalable platform technology enables optimal isolation, activation, and purity, yield, desired phenotype, cell viability of subpopulations of T-cells.CTS Detachable Dynabeads CD3/CD28CTS Dynabeads CD3/CD28CTS Treg XpanderDynabeads Human T-ExpanderCTS Detachable Dynabeads CD3/CD28The Gibco CTS Detachable Dynabeads CD3/CD28 Kit is a flexible and scalable platform technology that helps enable isolation and activation in one step while possessing a differentiating active release mechanism. CTS Detachable Dynabeads CD3/CD28 has been found to be comparable to the passive detachable Dynabeads in IL-2 expression, activation marker profile, cytokine release panel, and cell proliferation levels. The key difference is the added release functionality to address biological variability and helps provide users with flexibility in their manufacturing process.Click to enlargeFigure 1AClick to enlargeFigure 1BFigure 1.CTS Detachable Dynabeads CD3/CD28 induces similar expression of activation markers for A) CD69 and B) CD25 as the passive release CTS Dynabeads CD3/CD28. On days 2 and 3 post-activation, 94% to 98% of T-cells are expressing the CD25 activation marker, respectively.
Ordering information
A56992, A56993CTS Dynabeads CD3/CD28Used in more than 100 clinical trials, Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and/or expansion of T-cells ex vivo. Whether working with polyclonal T-cells, antigen-specific T-cells, gene-modified T-cells, regulatory T-cells, or other T-cell subtypes, CTS Dynabeads magnetic beads enable the recovery of T-cells with properties comparable to in vivo–activated T-cells. This one-step process of simultaneous T-cell isolation and activation yields pure and uniformly activated T-cells. Click to enlargeFigure 2. Activated T-cells typically expand >100-fold in 10 days. (A, B) Isolated and activated T-cells were expanded for up to 10 days, and both CD4+ and CD8+ T-cells proliferated. (C, D) On day 10 post-activation, there were mainly CD45RA+/– CD127+/– CD28+ CD62L+ cells with low PD-1 expression.
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40203DCTS Treg XpanderGibco CTS Dynabeads Treg Xpander provides an optimized activation signal for activation and expansion of Regulatory T-cells (Tregs) and yields high-fold expansion of suppressive Tregs with a stable Treg phenotype, which is essential for use of Tregs in clinical applications.Key featuresScalable technology for robust activation and expansion of TregsClinically relevant numbers of Tregs with >800-fold expansion after two stimulationsAseptically manufactured and ready for clinical trials and commercial manufacturingMaintained Treg phenotype (FoxP3) and suppressive functionDrug Master File availableConformity with cGMP guidelinesClick to enlargeFigure 3.Optimized activation signal expands Tregs to clinically relevant numbers. Flow sorted regulatory T-cells were expanded with CTS Dynabeads Treg Xpander, CTS Dynabeads CD3/CD28 or competitor technology for 14 days with restimulation at day 9.Click to enlargeFigure 4. Optimized activation signal expands Tregs with high suppressive potential. Magnetically isolated regulatory T-cells were expanded three times with CTS Dynabeads Treg Xpander for 14 days. Expanded Tregs are highly suppressive.
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46000DDynabeads Human T-Expander CD3/CD28 Dynabeads Human T-Expander CD3/CD28, the research-grade version of CTS Dynabead CD3/CD28, is intended for separation, activation, and expansion of human T-cells. Containing the same amount and ratio of antibodies from the same clones as CTS Dynabeads CD3/CD28, Dynabeads Human T-Expander CD3/CD28 are intended for small-scale pre-clinical research. Dynabeads Human T-Expander CD3/CD28 offers a simple method for the activation and expansion of T-cells that does not require antigen presenting cells or antigen.
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11141D
CTS Detachable Dynabeads CD3/CD28
CTS Detachable Dynabeads CD3/CD28The Gibco CTS Detachable Dynabeads CD3/CD28 Kit is a flexible and scalable platform technology that helps enable isolation and activation in one step while possessing a differentiating active release mechanism. CTS Detachable Dynabeads CD3/CD28 has been found to be comparable to the passive detachable Dynabeads in IL-2 expression, activation marker profile, cytokine release panel, and cell proliferation levels. The key difference is the added release functionality to address biological variability and helps provide users with flexibility in their manufacturing process.Click to enlargeFigure 1AClick to enlargeFigure 1BFigure 1.CTS Detachable Dynabeads CD3/CD28 induces similar expression of activation markers for A) CD69 and B) CD25 as the passive release CTS Dynabeads CD3/CD28. On days 2 and 3 post-activation, 94% to 98% of T-cells are expressing the CD25 activation marker, respectively.
Ordering information
A56992, A56993
CTS Dynabeads CD3/CD28
CTS Dynabeads CD3/CD28Used in more than 100 clinical trials, Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and/or expansion of T-cells ex vivo. Whether working with polyclonal T-cells, antigen-specific T-cells, gene-modified T-cells, regulatory T-cells, or other T-cell subtypes, CTS Dynabeads magnetic beads enable the recovery of T-cells with properties comparable to in vivo–activated T-cells. This one-step process of simultaneous T-cell isolation and activation yields pure and uniformly activated T-cells. Click to enlargeFigure 2. Activated T-cells typically expand >100-fold in 10 days. (A, B) Isolated and activated T-cells were expanded for up to 10 days, and both CD4+ and CD8+ T-cells proliferated. (C, D) On day 10 post-activation, there were mainly CD45RA+/– CD127+/– CD28+ CD62L+ cells with low PD-1 expression.
Ordering information
40203D
CTS Treg Xpander
CTS Treg XpanderGibco CTS Dynabeads Treg Xpander provides an optimized activation signal for activation and expansion of Regulatory T-cells (Tregs) and yields high-fold expansion of suppressive Tregs with a stable Treg phenotype, which is essential for use of Tregs in clinical applications.Key featuresScalable technology for robust activation and expansion of TregsClinically relevant numbers of Tregs with >800-fold expansion after two stimulationsAseptically manufactured and ready for clinical trials and commercial manufacturingMaintained Treg phenotype (FoxP3) and suppressive functionDrug Master File availableConformity with cGMP guidelinesClick to enlargeFigure 3.Optimized activation signal expands Tregs to clinically relevant numbers. Flow sorted regulatory T-cells were expanded with CTS Dynabeads Treg Xpander, CTS Dynabeads CD3/CD28 or competitor technology for 14 days with restimulation at day 9.Click to enlargeFigure 4. Optimized activation signal expands Tregs with high suppressive potential. Magnetically isolated regulatory T-cells were expanded three times with CTS Dynabeads Treg Xpander for 14 days. Expanded Tregs are highly suppressive.
Ordering information
46000D
Dynabeads Human T-Expander
Dynabeads Human T-Expander CD3/CD28 Dynabeads Human T-Expander CD3/CD28, the research-grade version of CTS Dynabead CD3/CD28, is intended for separation, activation, and expansion of human T-cells. Containing the same amount and ratio of antibodies from the same clones as CTS Dynabeads CD3/CD28, Dynabeads Human T-Expander CD3/CD28 are intended for small-scale pre-clinical research. Dynabeads Human T-Expander CD3/CD28 offers a simple method for the activation and expansion of T-cells that does not require antigen presenting cells or antigen.
Ordering information
11141DRelated ResourcesApplication notesInnovating for the future: Accelerating the arrival of immuno-oncology 2.0Integrated generation and characterization of CAR T cellsOne-step isolation and activation of naive and early memory T cells with CTS Dynabeads CD3/CD28Successful support of longer T cell expansion workflows with CTS Dynabeads and CTS Optmizer Expansion SFM, no phenol redCTS Detachable Dynabeads CD3/CD28 Kit provides one-step isolation and activation with flexible active release to optimize T cell phenotypeFlyer Meet the next generation of CTS Dynabeads magnetic beads
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Cell Culture & Transfection Learning CenterCell Culture & Transfection Support CentersDesigned and manufactured to provide quality and consistencyOur Gibco, Thermo Scientific Nunc, and Invitrogen cell culture products help you to achieve reproducible results and successful research outcomes—every day.Cell Culture MediaDMEMDMEM/F12MEMRPMI 1640& MoreFetal Bovine SerumValue FBSPremium FBSSpecialty FBSOther Animal Sera& MoreCell Culture PlasticsFlasksDishesPlatesPipettes& MoreTransfection ReagentsPlasmid DNAsiRNAin vivomRNA& MoreFind how Gibco Media, FBS & Nunc Plastics are better together ›
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Find present research applications and proof-of-concept experiments involving various products from our cell biology portfolio, including Thermo Scientific, Invitrogen and Gibco products. Browse our protocols for guidelines and general procedures for routine sub-culturing, thawing, and freezing of cells in culture.Application Notes | Cell Culture Protocols | Transfection Protocols Cell Culture Support Center | Transfection Support Center
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Find the right cell culture products for your experiment using the selection guides below.Media Formulation Tool: Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM and RPMI-1640 media.iMatch Sera Lot Matching Tool: Our FBS specialists utilize iMATCH to find you the most consistent or highest performing lot of serum available.Nunc Selection Tool: Switch to Nunc cell culture plastics with this convenient cross reference tool.Transfection Reagent Selection Tool: Choose from a range of delivery options to best suit your transfection experiment
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Whether you are looking for tips and tricks or need an introduction to cell culture, look no further than our Gibco Cell Culture Basics where we provide basic cell culture techniques and methods for passaging, freezing, and thawing cultured cells.Start here if you are new to cell culture techniques or need to refresh your knowledge
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Gibco Cell Culture Heroes spotlights PhD and postdoc researchers across the globe with the primary focus of telling the story of their research. This program promotes education and creates a community amongst researchers who are driving tomorrow's breakthroughs using cell culture.Learn more GMP manufacturingGibco cell culture products are manufactured in facilities that are compliant with current good manufacturing practices (GMP) for medical devices and adheres to a robust quality management system.Learn more about GMP manufacturing Sustainable solutionsWe are committed to delivering products that serve the research needs of our customers, while striving to develop them in a way that minimizes our use of natural resources and our impact on the environment.Learn more about sustainable solutions
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Cell Culture & Transfection Learning Center Access cell culture and transfection educational resources for better experiment planning and execution.Media Formulation Tool Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.Related productsCell culture mediaCell culture plasticsCell culture FBSCell culture reagentsCell culture instruments & equipmentTransfection products
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Cell Culture & Transfection Support Center Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.Need technical support? Contact our expert team for technical and application support of Laboratory ProductsFor Research Use Only. Not for use in diagnostic procedures.
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Home›Life Sciences›Bioprocessing›Gibco Cell Culture for Bioprocessing›Gibco Media by Design ServicesGibco Media by Design ServicesSee Navigation‹Gibco Cell Culture for BioprocessingGibco CHO Media
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›Freedom cell line development kitsMedia and feed panelsCustom media developmentBioprocessing analyticsGibco Rapid PrototypingUnique goals. Customized solutions. Success by design.From antibodies to advanced therapies, collaborate with the knowledgeable and trusted pioneer of bioprocessing innovation, providing customized solutions that help empower you to meet your project goals.Contact us Download brochure
Gibco Media by Design Services
Previously known as "PD-Express Services"Freedom Kits for stable clone developmentGibco Freedom™ cell line development kits are designed to provide you with integrated, stable cell line development solutions to simplify your development workflow and accelerate your journey to commercial manufacturing.Media and feed evaluation panelsAccelerate your bioprocessing success with diverse, ready-to-use formulations for simplified evaluation. Ready-to-use prototype formulations (panel libraries) are developed specifically for CHO or HEK293 cells.Custom medium and feed development servicesMeet your upstream process goals with optimized formulations, developed through collaboration. Our media development service is available in traditional (spent media DOE) and multi-omics workflow options.Bioprocessing analyticsGain actionable insights powered by industry-leading technology and trusted expertise. Media analytics, protein analytics and investigative analytics can potentially help get the most from your cell culture and shorten time to market.Gibco Rapid Prototyping serviceAccelerate media development and scale up with fast, non-GMP custom media manufacturing at pilot scale. Test the manufacturability and scalability of your formulation or modify existing formulations.Catalog media evaluationUtilizing our extensive portfolio of catalog products, we can provide you with off-the-shelf solutions that are ready to ship, helping you save time, effort, and costs for your unique production requirements.Unsure which service might be right for you? Try our Media by Design™ Services interactive selection guide. Select your modality and challenge, learn about options available for your specific needs.Services Selector
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Review our case studies and white papers to learn more about how Gibco Media by Design Services can help address your process development challenges. Investigative analyticsAchieving consistent performance by identifying key drivers in media and supplementsDownloadCustom media developmentCustom traditional and multi-omics media development enhances antibody production in CHO cells.DownloadGibco Rapid PrototypingSuccessful manufacturing of dry powder media from development to large-scale cGMP production.DownloadDiscover your advantageOur process development services vary based on your exact needs but share the following attributes. Personalized: Gibco Media by Design Services deliver flexible solutions to help meet your unique project needs, tailored through collaboration. Dependable: We provide reliable expertise (over 60 years of cell culture experience) and trusted insights to help you achieve your process goals—at every stage and scale.Advanced: Our experienced teamoffers innovative solutions and has the knowledge to support process development for emerging modalities and workflows.Additional servicesMedia manufacturing services: Tailored cGMP large-scale media manufacturing services to suit your needs, no matter the developmental stage. We also offer Media Customization Services for our catalog products. Discover how we can accelerate your manufacturing journey.Trace element analysis and testing services: Increase control and visibility to the trace element profile of your cell culture media and feeds. Proactively track historical trends and create predictive media formulations, all with a goal to reduce lot-to-lot variability.
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HomeShop All ProductsCell Culture Media, Supplements, and ReagentsCell Culture MediaClassical and Basal Cell Culture MediaDMEM, high glucoseGibco™DMEM, high glucoseCatalog number: 11965118Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceGibco™DMEM, high glucoseCatalog number: 11965118Related applications: Mammalian Cell CultureTechnical SupportCustomer ServiceCatalog Number11965118also known as 11965-118Unit Size10 x 500 mLPrice (USD)Contact Us ›Save to listCatalog Number11965092also known as 11965-092Unit Size500 mLPrice (USD)Contact Us ›Save to listCatalog Number11965084Unit Size1,000 mLPrice (USD)Contact Us ›Save to listCatalog Number11965126Unit Size6 x 1,000 mLPrice (USD)Contact Us ›Save to listCatalog Number11965167also known as 11965-167Unit Size5 LPrice (USD)Contact Us ›Save to listCatalog Number11965175also known as 11965-175Unit Size10 LPrice (USD)Contact Us ›Save to listCatalog NumberUnit SizePrice (USD)11965118also known as 11965-11810 x 500 mLContact Us ›11965092also known as 11965-092500 mLContact Us ›119650841,000 mLContact Us ›119651266 x 1,000 mLContact Us ›11965167also known as 11965-1675 LContact Us ›11965175also known as 11965-17510 LContact Us ›Save to listCustomize this productProduct OverviewFiguresVideosRecommendationsRecommendationsDocumentsFAQCitations & ReferencesAdditional InformationRecommendationsDMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This DMEM is modified as follows:WithWithout• High Glucose• Sodium Pyruvate• L-glutamine• HEPES• Phenol RedThe complete formulation is available.Using DMEMDMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.SpecificationsCell LineHeLa, 293, Cos-7, and PC-12Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle CellsClassificationAnimal Origin-freeConcentration1 XFormLiquidProduct TypeDMEM (Dulbecco's Modified Eagle Medium)Serum LevelStandard Serum SupplementationShelf Life12 Months From Date of ManufactureSterilitySterile-filteredWith AdditivesHigh Glucose, Glutamine, Phenol RedWithout AdditivesNo HEPES, No Sodium PyruvateFor Use With (Application)Mammalian Cell CultureGlucose Concentration4500 mg/LGreen FeaturesSustainable packagingManufacturing QualitycGMP-compliant under the ISO 13485 standardProduct LineGibco™Quantity10 x 500 mLShipping ConditionRoom TemperatureContents & StorageStorage conditions: 2°C to 8°C (protect from light)Shipping conditions: AmbientShelf life: 12 months from date of manufactureFiguresDocuments & DownloadsCertificatesSearch by lot number or partial lot numberSearchRequest a CertificateFrequently asked questions (FAQs)Citations & ReferencesSearch citations by name, author, journal title or abstract textSearch
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Gibco®细胞培养基本知识在线视频 | Thermo Fisher Scientific - PH
Gibco®细胞培养基本知识在线视频 | Thermo Fisher Scientific - PH
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Home›Gibco®细胞培养基本知识在线测验›Gibco®细胞培养基本知识在线视频See Navigation
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细胞培养基本知识视频：
视频1：细胞培养简介x本视频概述了细胞培养中的一些基本设备及正确的实验室布局。视频2：无菌技术x本视频重点介绍了细胞培养过程中为避免污染所应遵循的步骤。视频3：细胞传代x本视频讲述了为什么，何时及怎样对贴壁细胞和悬浮细胞进行传代。视频4：冻存细胞x本视频展示了冻存细胞的关键步骤以保持理想的细胞活力。视频5：复苏细胞x本视频介绍了避免损伤细胞的正确复苏方法。
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同样是细胞培养基，GIBCO的为什么比Hyclone和wisent的贵那么多？ - 知乎
同样是细胞培养基，GIBCO的为什么比Hyclone和wisent的贵那么多？ - 知乎首页知乎知学堂发现等你来答切换模式登录/注册生物学细胞生物化学同样是细胞培养基，GIBCO的为什么比Hyclone和wisent的贵那么多？同样是一瓶500ML的DMEM，Gibco的价格为250左右，Hyclone和wisent却只要50-60元，中间的差价是因为什么？难道仅仅是品牌效应…显示全部 关注者29被浏览112,726关注问题写回答邀请回答好问题添加评论分享9 个回答默认排序知乎用户教师资格证持证人自己两种都用过。感觉gibco的好用。养的一些细胞，娇气一点的一碰hyclone就死编辑于 2015-09-17 11:47赞同 1添加评论分享收藏喜欢收起知乎用户产地不同。你买gibco直口那种，进口的粉国内生产线冲了灌装的，和hyclone差不多。因为hyclone也是国内灌装的。歪瓶口那种是国外整瓶进口的，运费海了去了，所以贵。另外gibco的贵确实是整个品牌的价值高一点点，所以thermo收购life technologies后卖掉了hyclone，甩给GE了。另外有个小道，据说gibco控制了澳洲血源，因此澳洲血源的血清，最紧张的时候hyclone根本没有。编辑于 2015-09-10 22:51赞同 1410 条评论分享收藏喜欢